MAN5, a Glycosyl Hydrolase Superfamily Protein, Is a Key Factor Involved in Cyanide-Promoted Seed Germination in Arabidopsis thaliana

Seed germination is the complex adaptive trait of higher plants influenced by a large number genes and environmental factors. Numerous studies have been performed to better understand how controlled various factors applied chemicals, such as cyanide. However, still very little known about molecular mechanisms extrinsic signals regulate seed germination. Our previous found that non-lethal cyanide treatment promotes germination, but regulatory mechanism unclear. In this study, we low concentration pretreatment significantly enhanced expression endo-β-mannanase 5 (MAN5) gene in Arabidopsis thaliana, mutation impaired cyanide-mediated contrast, overexpression MAN5 ability under both normal salt stress conditions. Further showed was negatively regulated ABA insensitive (ABI5); abi5 mutant seeds, increased rate accelerated. Additionally, markedly reduced ABI5 seeds. Taken together, our data support involvement key confirm role critical negative factor involved cyanide-regulated expression.